Título

Frequency of gene polymorphisms of Proinflammatory and anti-inflammatory cytokines in a population from Parana, Southern Brazil.

Introdução

A number of proinflammatory and anti-inflammatory cytokines have been identified as key components in the pathogenesis of many diseases including infectious diseases, cancer, autoimmunity, and inflammatory conditions. Polymorphisms of several cytokine genes located within the promoter or other regulatory regions may affect the gene transcription, and influence the level of production of cytokines, conferring flexibility in the immune response. The genetic constitution of the Brazilian population presents peculiarities that vary according to region. The aim of this study was to estimate the frequency of cytokine gene polymorphisms in a mixed population from Parana state, in the South region of Brazil.

Material e Método

Single nucleotide polymorphisms (SNPs) were genotyped in genes encoding proinflammatory (IL-12, IFN-γ and TNF-α) and anti-inflammatory (TGF-β1, IL-4 and IL-10) cytokines, in 350 subjects from Parana, Brazil, by PCR-SSP using Kits of Invitrogen®.

Resultados

The allele distribution was consistent with the Hardy-Weinberg equilibrium. The allele frequencies of proinflamatory cytokine polymorphisms were: IL12-1188, (C: 0.71 and A: 0.29), IFNG+874 (A: 0.61 and T: 0.39), TNF-308 (G: 0.86 and A: 0.14),TNF-238(C: 0.95 and T: 0.05), and anti-inflammatory were: TGFB1+869 (T: 0.54 and C: 0.46), TGFB1+915 (G: 0.92 and C: 0.08), IL4-1098 (T: 0.86 and G: 0.34), IL4-590 (T: 0.69 and C: 0.31), IL10-1082 (G: 0.64 and A: 0.36), IL10-819 (T: 0.69 and C: 0.31), and IL10-592 (C: 0.69 and A: 0.31).

Discussão e Conclusões

This study showed the frequency of cytokine gene polymorphisms in a mixed population from Parana state, in the South region of Brazil, and it may contribute to a better understanding of genetic background and its role in diseases.

Palavras Chave

Cytokines, SNP, Genetic Background.